This week has been full of collecting data through the experiment known as the ELISA. ELISA stands for Enzyme-Linked ImmunoSorbent Assay. It's used for looking for antibodies. Here, the ELISA data can be used along with the Elispot data and the FACS data to prove that there is indeed an immune response taking place where they are supposed to.
On Monday, I did an ELISA looking at the immune responses with the sera of a few different mice in different dilutions, so as to determine which of these seras and at which particular dilution is good to serve as a control for the next few ELISAs.
On Tuesday, the ELISA utilized the sera of mice that were part of the same takedown that yielded the Elispot and FACS data on the spleen and peritoneal cavity over the last two weeks. Wednesday was a repeat of the same ELISA to check that the results are correct. Thursday, I did an ELISA using the sera from the other 3 mice part of the same takedown. With a combination of all these results, we're going to lab meeting to present the data and look for ideas and input from the other lab fellows on what to do next.
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